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Bioss標記抗體高分文獻引用精選


Ashok Kumar Jayavelu. et al. Splicing factor YBX1 mediates persistence of JAK2 -mutated neoplasms. Nature. 2020 Nov;588(7836):157-163.

Nature2020 Pubmed ID:33239784

產品編號Anti-JAK2/Cy3 (bs-0908R-Cy3)

IF: 42.778 Application: IF Species: Mouse


Zhai, Yihui. et al. T lymphocyte membrane-decorated epigenetic nanoinducer of interferons for cancer immunotherapy. Nat Nanotechnol. 2021 Sep;:1-10.

Nature Nanotechnology2021 Pubmed ID:34580467

產品編號:Anti-LAMP2/FITC (bs-2379R-FITC)

IF: 39.213 Application: IF Species: Mouse


Alivernini S et al. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis. Nat Med. 2020 Aug;26(8):1295-1306.

Nature Medicine2020 Pubmed ID:32601335

產品編號:Anti-MERTK/Cy3 (bs-0548R-Cy3)

IF: 36.13 Application: IF Species: Human


Emeric Limagne. et al. MEK inhibition overcomes chemoimmunotherapy resistance by inducing CXCL10 in cancer cells. Cancer Cell. 2022 Jan.

Cancer Cell2022 Pubmed ID: 35051357

產品編號:Anti-C-Myc/AF594 (bs-4963R-AF594)

IF: 31.743 Application: IF Species: Mouse


Dumont, Nicolas A., et al. "Dystrophin expression in muscle stem cells regulates their polarity and asymmetric division." Nature Medicine (2015).

Nature Medicine2015 Pubmed ID:26569381

產品編號:Anti-phospho-MARK2(Thr596)/AF647 (bs-5742R-AF647)

IF: 30.357 Application: IF Species: Mouse


Lu, Yiwen. et al. Eosinophil extracellular traps drive asthma progression through neuro-immune signals. Nat Cell Biol. 2021 Oct;:1-13.

Nature Cell Biology2021 Pubmed ID:34616019

產品編號:Anti-EAR1/AF555 (bs-1754R-AF555)

IF: 28.824 Application: IF Species: Human


Homan KA et al. Flow-enhanced vascularization and maturation of kidney organoids in vitro. Nat Methods. 2019 Mar;16(3):255-262.

Nat Methods2019 Pubmed ID:30742039

產品編號:Anti-VEGFR2/AF555 (bs-10412R-AF555)

IF: 28.467 Application: FCM Species: Human


Cai, Baohuan. et al. IRAK1-regulated IFN-γ signaling induces MDSC to facilitate immune evasion in FGFR1-driven hematological malignancies. Mol Cancer. 2021 Dec;20(1):1-20.

Molecular Cancer2021 Pubmed ID:34906138

產品編號:Anti-NKG2D/AF555 (bs-0938R-AF555)

IF: 27.401 Application: FCM Species: Mouse


Cheng Gao. et al. Surface-engineered chlorella alleviated hypoxic tumor microenvironment for enhanced chemotherapy and immunotherapy of first-line drugs. MATER TODAY. 2022 Jul.

Materials Today2022 Pubmed ID:10.1016/j.mattod.2022.06.024

產品編號:Anti-HIF-1 Alpha/AF555 (bs-0737R-AF555)

IF: 26.943 Application: IF Species: Mouse


Eszter á. Tóth. et al. Formation of a protein corona on the surface of extracellular vesicles in blood plasma. J Extracell Vesicles. 2021 Sep;10(11):e12140.

Journal of Extracellular Vesicles2021 Pubmed ID: 34520123

產品編號:Anti-Fibrinogen alpha chain/FITC (bs-7548R-FITC)

IF: 25.841 Application: Immune electron microscopy Species: Human


Li, Fengyin, et al. "Prostaglandin E1 and Its Analog Misoprostol Inhibit Human CML Stem Cell Self-Renewal via EP4 Receptor Activation and Repression of AP-1." Cell Stem Cell 21 (2017): 1-15.

Cell Stem Cell2017 Pubmed ID:28844837

產品編號:Anti-EP3/PTGER3/AF647 (bs-1876R-AF647)

Anti-Prostaglandin E Receptor EP1/AF647 (bs-6316R-AF647)

IF: 23.394 Application: FCM Species: Mouse


Yamamizu, Kohei, et al. "Protein Kinase A Determines Timing of Early Differentiation through Epigenetic Regulation with G9a." Cell stem cell 10.6 (2012): 759-770.

Cell Stem Cell》2012 Pubmed ID: 22704517

產品編號:Anti-FOXA2/PE (bs-2358R-PE)

Anti-Nestin/PE (bs-0008R-PE)

IF: 22.634 Application: FCM Species: Mouse


Ni L et al. Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. Immunity 2020 06 16;526(6).

Immunity》2020 Pubmed ID:32413330

產品編號:Goat Anti-Human IgM / HRP (bs-0345G-HRP)

IF: 22.553Application: ELISA Species: Human


Shogo Kumagaiet al. An Oncogenic Alteration Creates a Microenvironment that Promotes Tumor Progression by Conferring a Metabolic Advantage to Regulatory T Cells. Immunity. 2020 Jul 14;53(1):187-203.e8.

Immunity》2020 Pubmed ID:32640259

產品編號:Anti-PPAR alpha/AF647 (bs-3614R-AF647)

Anti-PPAR Gamma/PE (bs-4590R-PE)

IF: 22.553 Application: FCM Species: Human


Lin Yao. et al. Nasal Spray of Neutralizing Monoclonal Antibody 35B5 Confers Potential Prophylaxis Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Variants of Concern (VOCs): A Small-scale Clinical Trial. CLIN INFECT DIS. 2022 Jun.

CLINICAL INFECTIOUS DISEASES》2022 Pubmed ID:35666466

產品編號:Goat Anti-Human IgG H&L / HRP (bs-0297G-HRP)

IF: 20.999 Application: ELISA Species: Human


Miller A et al. ""Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments."" Cell Metabolism(2017)."

Cell Metabolism》2017 Pubmed ID: 28889950

產品編號:Anti-FAPA/AF647 (bs-5758R-AF647)

Anti-CD31/AF488 (bs-0468R-AF488)

IF: 20.565 Application: IF Species: Human


van Ree JH et al. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. (2016) Nat.Cell.Bio. 18:814-21.

Nat Cell Biol》2016 Pubmed ID:27240320

產品編號:Anti-SLC33A1/FITC (bs-0699R-FITC)

IF: 20.06 Application: IF Species: Mouse





標記抗體服務



服務宗旨:高品質的標記原料采購,規范的標記操作流程,嚴格的標記產品質控,合理的市場價格定位。
服務目標:以國際高標準的標記服務水平,提供高品質的標記抗體產品,助力科研人員輕松科研。



一、服務內容:

本服務項目主要為接收客戶的抗體、蛋白、多肽、小分子化合物進行常規標記物標記。

1. 可接收的待標記樣品包含抗體IgG、大分子蛋白、小分子多肽、小分子化合物等。

2. 可提供標記服務的標記物有:

A. 酶標記物:堿性磷酸酶(AP/ALP),辣根過氧化物酶(HRP);

B. 生物素(Biotin);

C. 常規熒光素:FITC,Rhodamine B(RBITC);

D. Cy系列熒光素:Cy3,Cy5,Cy5.5, Cy7;

E. 熒光蛋白:藻紅蛋白(PE),別藻藍蛋白(APC),PerCP;

F. 熒光雙標記:PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7;

G. 膠體金標記Co-Au;

二、服務流程:

1.  客戶提交委托標記請求并明確標記要求,與公司商定具體的實驗項目、數量和費用,雙方簽訂《委托標記制備合同》。

2.  客戶提供待標記樣品,我公司按照合同要求安排標記服務,并在規定時間內完成標記委托。

3.  標記完成后,我公司按照客戶要求分裝,免費低溫郵寄給客戶,同時提供標記報告。

三、樣品要求:

1.  待標記樣品需保證90%以上的純度,樣品中不含有干擾標記物結合的成分,如有特殊成分,請提前標注說明。

2.  待標記樣品最小標記量為1mg,最好是干粉,如果是液體,濃度應大于1mg/ml。

3.  抗體IgG樣品中應不含BSA、疊氮鈉、甘氨酸等成分。

4.  大分子蛋白應提供分子量、等電點、緩沖液成分及pH等信息;小分子多肽需提供氨基酸序列;小分子化合物還需提供分子結構式。

5.  請提前說明任何特殊情況。

四、收費標準:

1.  抗體和大分子蛋白標記收費標準:

標記物 類型 標記數量 周期
0.5-1mg 1-2mg
2-5mg
HRP 1200 1600 2500 10天
AP 2400 3600 6400 10天
Biotin 生物素 1200 1600 2500 10天
FITC 熒光 1200 1600 2500 10天
RBITC 熒光 1200 1600 2500 10天
Cy系列 熒光 1500 2200 3200 10天
AFluor 熒光 2400 3600 6400 10天
PE 熒光 2400 3600 6400 10天
APC 熒光 2400 3600 6400 10天
PerCP 熒光 3000 4800 - 10天
PE-Cy雙標 熒光 3000 4800 - 15天
Co-Au 膠體金 1500 2500 4000 10天

五、標記物介紹:
堿性磷酸酶(alkaline phosphatase,ALP或AP)是免疫診斷試劑產品最常用的標記酶之一,優點是穩定性高、靈敏度高。ALP是一種能夠將對應底物去磷酸化的酶,即通過水解磷酸單酯將底物分子上的磷酸基團除去,并生成磷酸根離子和自由的羥基,這類底物包括核酸、蛋白、生物堿等。而該脫去磷酸基團的過程被稱為去磷酸化或脫磷酸化。堿性磷酸酶是磷酸酶的一種,磷酸酶的作用與激酶的作用正相反,激酶是磷酸化酶,可以利用能量分子,如ATP,將磷酸基團加到對應底物分子上。

辣根過氧化物酶(Horseradish Peroxidase,HRP)活性高,穩定,分子量小,純酶容易制備,所以最常用。HRP廣泛分布于植物界,辣根中含量高,它是由無色的酶蛋白和棕色的鐵卟啉結合而成的糖蛋白,糖含量18%。HRP由多個同功酶組成,分子量為40,000,等電點為PH3~9,酶催化的最適PH因供氫體不同而稍有差異,但多在PH5左右。酶溶于水和58%以下飽和度硫酸銨溶液。HRP的輔基和酶蛋白最大吸收光譜分別為403nm和275nm,一般以OD403nm /OD275nm的比值RZ(德文ReinheitZahl)表示酶的純度。高純度的酶RZ值應在3.0左右(最高可達3.4)。RZ值越小,非酶蛋白就越多。

FITC和RhodamineB(RBITC)是常見的熒光素,性質較穩定;

Cy系列熒光染料由于熒光較強,對光猝滅有抵抗力而熒光不宜消退,近年來應用比較廣泛;





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